Journal: Molecular cancer research : MCR

Article Title: HD Chromoendoscopy Coupled with DNA Mass Spectrometry Profiling Identifies Somatic Mutations in Microdissected Human Proximal Aberrant Crypt Foci

doi: 10.1158/1541-7786.MCR-13-0624

Figure Lengend Snippet: A, DNA-MS identification of an insertion/deletion to the EGFR gene in a serrated hyperplastic proximal colon ACF. B, DNA-MS detection of a G>C missense mutation resulting in FLT3I836M somatic mutation in a distended hyperplastic distal colon ACF. UEP=unextended primer

Article Snippet: Samples were then cleaned using the DNA Wizard protocol (Promega, Madison, WI) Somatic mutation screening using DNA mass-spectrometry Mutation screening was performed at the Yale Center for Genome Analysis using the Sequenom MASSArray DNA-MS approach.

Techniques: Mutagenesis

Journal: Molecular cancer research : MCR

Article Title: HD Chromoendoscopy Coupled with DNA Mass Spectrometry Profiling Identifies Somatic Mutations in Microdissected Human Proximal Aberrant Crypt Foci

doi: 10.1158/1541-7786.MCR-13-0624

Figure Lengend Snippet: A, DNA-MS reveals a c.2626C>T substitution resulting in an APCR876* mutation in a proximal dysplastic ACF. UEP = unextended primer. B, IHC demonstrates an accumulation of nuclear β-catenin (black arrowheads) in dysplastic crypts positive for the APCR876* mutation compared to adjacent normal crypts. (400× magnification; bar = 30 µm) C, Sanger sequencing confirms that the APCR876* mutation is somatically acquired. Blood and synchronous tubular adenomas are wild-type at codon 876 of APC.

Article Snippet: Samples were then cleaned using the DNA Wizard protocol (Promega, Madison, WI) Somatic mutation screening using DNA mass-spectrometry Mutation screening was performed at the Yale Center for Genome Analysis using the Sequenom MASSArray DNA-MS approach.

Techniques: Mutagenesis, Sequencing